Sprout: Character 1: Sprout is a strawberry who wears solely a white scarf with thin pink stripes wrapped around his neck and draped over his back. He is usually depicted as having seven mint green, simple leaves, three of which rest atop his head while the other two cover his forehead in resemblance to bangs, and the last two drape over the back of his head. Sprout's Appearance adds one more leaf onto his front bangs, and he has dark red blush complemented with three black freckles on each cheek, which adds up to a total of 6 freckles. His limbs are a vivid shade of red, being lighter than his torso and head.

Sprout: Character 1: Sprout is a straightforward, overprotective, and pragmatic Toon, as shown in his Passive Ability, being coined as "Overprotective". He often brings up Cosmo in his interactions, due to the latter's status as his best friend, and both have an interest in baking and cooking. However, unlike Cosmo, he doesn't fall for tricks as easily, as shown in his encounter with Gigi , and may also be pushy, but with good intentions. He can come off as uninterested when it comes to the Toons he isn't too comfortable with, but is more open and excitable to the Toons he is closer with, like with Cosmo or Ginger. Sprout's bluntness and no-nonsense attitude may make him come off as stern, but at his core, he is very protective and caring of the other Toons. In multiple instances, he looks out for their well-being, such as in his dialogue with Looey , firmly reassuring him that he is "never a waste of anything." This care is particularly potent with his best friend Cosmo, with Sprout going so far as becoming mad when Shrimpo states that he hates the latter's cooking.

Vee: Character 2: Vee is a pistachio green TV with a black screen. She has two black antennas topped with grey spheres, with her right antenna being bent in a sort of zigzag line while the other is straight. Her facial features are lime green with a confident smirk on her face. Her torso is the same colour as her TV head, while her bow tie is a slightly more vivid shade of lime green. Her limbs are a dark grey and, as seen in some artworks and her idle animations, she is revealed to have a retractable tail with a microphone topper. The microphone has a dark green handle with a grey grill. During Blackouts, she gives off a green glow. When hit by a Twisted, a blue error screen appears on her, which her glow and attacked face changes depending on what skin she's wearing.

Vee: Character 2: Vee is a straightforward and blunt Toon who sometimes acts arrogant and superior. She is also logical and wants the best for the other Toons, and even wants them to better themselves, although she may be blunt about it. Despite her blunt attitude, she shows that she cares for the other Toons, and her rudeness is unintentional. Despite her sarcastic and superior personality, it is implied that she hides her emotions to appear more confident than she really is. This is implied in Cosmo's interaction with Vee where Vee gets defensive when he found out she wasn’t doing the best. Vee is a sentient Toon and dislikes when she is not seen as more than her code or as if she doesn’t have emotions, such as when Rodger was planning to use Vee's knowledge for something. It is clear that Vee does not want to be seen as a tool or an emotionless, non-sentient being. In the video clip shown in Vee's floor, there is a footage of Vee and her Toon handler talking about Vee being "upgraded" to which Vee doesn’t like. This further shows Vee's insecurities. Though Vee comes off as straightforward and blunt, she hints that she cares for the other Toons and wants the best for them. It's also implied that her rudeness is unintentional.

Shelly: Character 3: Shelly is a beige ammonite shell with a black opening seen on the left side of her head. She has orange blush and two eyelashes underneath her eyes. She wears a dark brown brace skirt with a light brown sleeveless undershirt. When hurt, her eyes squint closed, and her smile turns into a grimace.

Shelly: Character 3: Shelly is an extroverted Toon, who enjoys talking about her interest in dinosaurs and other forms of prehistoric life, but she has other hobbies as well, such as sewing (which is shown in her dialogues with Bobette , Sprout , and Yatta ). She is highly supportive and kind towards others, as hinted at through her Active Ability, possibly due to the influence of her Toon Handler or natural energy. She's often surprised and even excited when other Toons show an interest in her (especially Main Characters), demonstrated in her dialogues with Astro , Vee , Yatta, Rodger , and Bassie Render , possibly because she often goes unnoticed or ignored by the others. For unknown reasons, Shelly is the most ignored Toon out of all the Main Characters, and this has been implied multiple times in the game. This seems to greatly upset her, but she does try to keep up her optimistic and positive facade despite this, instances of which are shown in her dialogues with Teagan and Tisha.

Tisha: Character 4: Tisha is a cyan tissue box that wears a dark blue bell-shaped dress with a light blue, rounded trim on the hem. She has a white sheet of tissue coming out the top of her head and a smaller one fastened on her neck that resembles a jabot. Her face has 9 blue freckles, and she often holds her hands behind her back when idling or running. In her Info Card and ability animation, she wields a small wooden Feather Duster with a black handle and deep brown feathers with light brown edges. When hurt by a Twisted, she has a somewhat worried look on her face.

Tisha: Character 4: Tisha is a Toon who loves cleaning, and dislikes anything that's deemed messy and/or disorganized—so much so that she can sometimes come off as obsessed. This can be backed up, as evidenced by her interaction with Boxten : "Shining, sparkling, so clean you'll need a wet floor sign!" She possibly cleans too much and overworks herself, as implied in her interaction with Astro and with Razzle & Dazzle. Despite her love of cleaning, she takes great offense and often gets quite frustrated with people not respecting the work she does, to the point where she'd either refuse cleaning requests, as shown in her interaction with Sprout, or outright show a dislike towards Toons that she deems not to appreciate her work enough, as shown in her interactions with Vee. However, she does show a more friendly side of herself to Shelly Render , as Tisha helps her clean fossils, showing she is more than just her fixation and will support the ones she cares about when needed. And it is revealed that her friendship with Brusha is one-sided.

Delilah Keen Room Handler: Character 5: Delilah has below waist length, ash brown hair with streaks, two sidelocks framing her face, low bangs and pale ivory skin. She is seen wearing a pair of dulled rainbow-themed pants and a white lab coat with light pink from lighting, both with black (presumably Ichor) stains on the cuffs and sleeve. She also appears to be wearing baby blue rubber gloves. Her smile is large and somewhat eerie in comparison to any of the other characters, whether human or Toon.

Delilah Keen Room Handler: Character 5: It seemed that Delilah's job was responsible for the creation of Toons; Ichor stains her clothes, and her equipment is that of a stereotypical scientist. By the notes scattered behind, it could also be assumed she had a passionate attitude in her work as Arthur doted on her a lot and was likely a workaholic too due to her dependency on coffee (there's even a coffee stain on one of the notes). Unlike Walton, she seemed to hold less ideals than him, coming off as tunnel-visioned, realist-minded and even greedy, brushing off his worries for a potential serious accident or that the Holiday Toons would be completely fine in solitude until their holidays hit again just to save on resources, without considering the psychological impact it would cause on their mental states (implying that she cared more about funding Gardenview and earning money than getting therapy or psychological help for the Toons).