Girlfriend: Girlfriend is the deuteragonist of Friday Night Funkin'. She is Boyfriend's partner and the daughter of Daddy Dearest and Mommy Mearest. She is usually seen sitting on top of a stereo, bopping her head to the tempo of the music, cheering Boyfriend on, and keeping track of combos. She is the first opponent faced as she teaches the player how to play the game with her only track, Tutorial. Girlfriend is a young 19 year old woman with light skin and long, thick brown-auburn hair. Two short locks of hair stick out of her head, one being much smaller than the other, and the shine on her hair resembles a skull. She wears a sleeveless red minidress and matching high heels. Her fingernails are painted red, as shown in her singing animations, where she points with both hands in the direction of the arrow note being hit, although sometimes this detail is not added. She normally sits with her legs crossed on a large gray speaker, with two smaller gray speakers laying beside the large one. She is drawn in a chibi style. Girlfriend is portrayed as a young, mysterious woman. With a love for cherry soda and her boyfriend, she seems to be calm and collected in almost any situation, even in the face of death. Being a demon, she seemingly possesses a great deal of power, but she mostly chooses to hide it. She is always seen with Boyfriend at her side, courtesy of true love, as she genuinely loves him very much. Like Boyfriend, Girlfriend lacks intelligence to the point of being unafraid of almost anything. In general, she either does not realize when she is in danger or is confident that she can get out of such situations, which may also be due in part to the power she has. Her lack of intelligence can result in her getting into difficult situations, such as getting her hand stuck in peanut butter jugs and having to wait for hours for someone to help get it out. However, she is still capable of making smart decisions and knows what to do when dealing with taxes[8] and facing death. Girlfriend has been diagnosed with attention deficit hyperactivity disorder (ADHD) and autism. The only individual she has been shown speaking to is Boyfriend. In Week 6 and Week 7, she does not speak at all, and she seems to barely acknowledge the opponent, if at all. Furthermore, she occasionally has poor object permanence. Girlfriend's talents as an artist and painter show that she is a creative individual. She has a poor concept of time, too, which is a common ADHD trait. She doesn't know very well how to handle money, either; she will normally make commissions (due to being an artist), but she will ask for 1 trillion dollars, or when she has to pay for a hamburger, she will pay less than what the hamburger costs.[12] Girlfriend has been described as being mean "in her own special way." She also likes to do immoral things, appearing to not care about the consequences of her actions as long as she gets what she wants. For instance, using her mother's limousine, she and Boyfriend hold up McDonald's drive-through slots as they hold the store hostage to get whatever food they want. She is the girlfriend of Boyfriend and her parents are Mommy Mearest and Daddy Dearest. She also has a demon form.

Nene: Nene is an asian 19 year old young woman with light skin and shoulder-length, bluish black hair. Two short locks of hair stick out of her head, one being much thinner than the other. Two longer strands of hair fall from her front hairline, each one parted to the side. The overall style of her hair is pointed, with multiple spikes on each side, particularly by her shoulders. She has a constant blush, razor-sharp teeth, and white eyes with no pupils. She wears a pink headband, a pinkish-white long-sleeved shirt under a pink overall minidress with heart-adorned straps, pinkish-white leggings, and pink shoes with medium-light red hearts adorning the sides. She is drawn in a chibi style. Nene sits atop a large green audio visualizer with speakers detached from A-Bot's body. The audio visualizer's main body is A-Bot's torso, which is medium-dark green and shiny. The front has a large display with a medium-light green outline around it and a semicircular notch centered at the bottom of the screen, with the capital letter A on the notch in medium-light yellow-green. The screen displays segmented bars in multiple colors on a very dark blue background. The conical speakers on the upper sides of the visualizer are gray with red inner grills and attached to darker bluish gray wheels; these cylinders are A-Bot's shoulders. Each arm wheel has a hole in the bottom. The left wheel has cut wires coming out of the hole and a cut tube coming out of the side, with rubble on the ground below the wires, all in the same shade of bluish gray. A-Bot's head is on the ground beside the audio visualizer, partly behind his right wheel. In her idle animation, Nene swings her left leg up and down, sticks her tongue out, and waves around a bloody, partially serrated knife in her right hand as she rests her left hand and right foot on the audio visualizer and bops her head to the tempo of the music. She wears a somewhat malicious and smug smile. Nene is depicted as a depressed, highly suicidal individual, particularly in the Pico series. In Friday Night Funkin' she maintains suicidal tendencies with her favored method being through a noose, but she always fails to tighten the noose enough to hang herself properly. The more she hangs out with Pico, the less she tries to end her own life. She also has promiscuous tendencies, as well as being a sadist and a masochist. Similarly to Darnell, she appears to be very greedy and selfish, going as far as to attack her own friend Pico for the sake of money. According to Pico's World, she has attention deficit hyperactivity disorder predominantly inattentive (ADHD-PI), meaning her sustained attention is poor. She is also lustful, as she tends to sleep around. She appears to have a penchant for knives in Friday Night Funkin'. She can also be quite mean. She is friends with Darnell and has a crush on Pico.

Cassandra: She has light skin, a mole on her right cheek, shoulder-length red hair with horn-like points at the front, with a shorter point situated between two that are taller, white eyes with no pupils, and sharp teeth. She wears a red sweater underneath a black jacket, along with black boots with red soles, gray plates on the toe caps, and bright yellow upside-down crosses on the sides. She also has long, sharp red nails and black ear piercings. She wields a TEC-9 in her right hand. She is drawn in a chibi style. Cassandra is a furry and also a rascal and gets angry very easily. She is teasing and has anger issues. Like Nene, she is kind of psychotic. She also has a Pennilian form. She is 19 years old.

The three of them are robots.